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Fig. 5. SDS-PAGE (A) and immunoblotting (B,C) patterns of blood solution fractions
obtained by hemin–agarose affinity chromatography. Immunoblotting used
antisera specific to mWap65-1 (B) and to mWap65-2 (C). Lanes 1, blood solution
of 2.8 mg ml–1 obtained from adult medaka; lanes 2,
supernatant; lanes 3, eluted fraction; lanes 4, proteins still bound to
hemin–agarose after elution; M, molecular mass markers (kDa). 10 µl
solution/lane. Arrowheads, see Fig.
4.
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