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Fig. 4. SDS-PAGE (A) and immunoblotting (B,C) patterns for recombinant proteins mWap65-1 (rWap65-1) and mWap65-2 (rWap65-2), and cytosolic protein fraction (8.0 mg ml–1) and blood solution (2.8 mg ml–1) from adult medaka. 10 µg of proteins were applied per lane and specific anti-rWap65-1 rabbit antiserum (B) and anti-rWap65-2 mouse antiserum (C) were used for immunoblotting. Lanes 1, rWap65-1; lanes 2, rWap65-2; lanes 3, a cytosolic protein fraction extracted from whole individuals of medaka; lanes 4, a blood solution obtained from adult medaka. M, molecular mass standards (kDa). The marks for rWap65-1 and rWap65-2 are as in Fig. 3 and the positions of intact mWap65-1 and mWap65-2 in SDS-PAGE gels are marked by red and blue arrowheads, respectively.





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