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Fig. 6. Effect of ISO on the relationship between SR Ca2+ uptake and the [Ca2+]i. (A) Representative intracellular Ca2+ recordings in control and with 1 µmol l–1 isoproterenol (ISO). Bars above traces indicate membrane potential (top bar) and extracellular solution (lower bar); –, regular bath solution; +, bath solution with 10 mmol l–1 caffeine. (B) Effect of membrane potential on the average Ca2+ transient during depolarization (black bars) and the peak Ca2+ transient elicited by 10 mmol l–1 Caf (white bars, N=7). (C) Effect of ISO on the relationship between SR Ca2+ uptake rate and [Ca2+]i (N=7). Solid (control) and dotted (ISO) lines represent fits of data with a Hill equation.





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