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Fig. 1. Experimental protocol. SR Ca2+ uptake was measured by clearance of the SR Ca2+ content by a brief exposure to 10 mmol l–1 caffeine (Caf) at –80 mV (Clear). The SR was then loaded during a 3 or 10 s depolarization (Load). The SR Ca2+ uptake during the depolarization to different membrane potentials (Vm) was quantified as the Caf releasable Ca2+ content and obtained by integration of the INCX elicited by Caf (Release).





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