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Fig. 6. Expression of ferritin heavy chain transcripts in hepatopancreas exposed to various conditions in vitro. Total RNA was isolated from the explanted hepatopancreas incubated under each condition and resolved on a 1.5% formaldehyde gel, blotted onto nitrocellulose and hybridized at 45°C to 32P-labeled probes produced from the ferritin heavy chain cDNA clone. Levels of mRNA transcripts are illustrated during normoxia (Con) and stress or second messenger exposure for paired hepatopancreas samples. The scanned intensity of experimental samples listed in the table is relative to the corresponding normoxic control. Values are means ± S.E.M. for N=3 independent hepatopancreas samples. aSignificantly different from control values as determined by the Student's t-test, P<0.01.





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