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Fig. 4. Ferritin heavy chain translation status measured using polysome profiles of
L. littorea hepatopancreas extracts prepared using post-mitochondrial
supernatants centrifuged on 15%–30% continuous sucrose density
gradients. Fractions were collected with high sucrose (30%) in fraction 1,
decreasing to 15% in fraction 15. Bar graphs show absorbance at 254 nm,
representing the relative amount of total RNA in each fraction. P, polysomes;
M, monosomes. Line graphs show ferritin heavy chain transcript levels as
determined from northern blots of total RNA isolated from each fraction. (A)
normoxia; (B) 72 h anoxia; (C) 6 h aerobic recovery following 72 h anoxia.
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