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Fig. 2. (A) Expression of ferritin heavy chain (HC) transcripts in hepatopancreas of L. littorea over a time course of anoxia exposure (12–120 h) and aerobic recovery (1 h after 120 h anoxia) as shown by northern blots (top). Scanned intensity of anoxic samples was normalized against the ethidium bromide (EtBr) stained gel (middle) and the 18S ribosomal RNA band (bottom) and then plotted relative to the intensity of the control band. (B) Histograms show means ± S.E.M. for N=3 independent blots using RNA extracted from different snails. aSignificantly different from the normoxic control as determined by the Dunnett's test, P<0.01. bSignificantly different from 1 h aerobic recovery, P<0.05. Con, control; R, recovery.





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