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Fig. 2. Confocal scanning of yolk-sac mitochondria-rich cells (MRCs) double-labeled
with Con-ATexas-Red (red) and Na+/K+-ATPase
antiserum (second antibody conjugated with FITC; green) in larvae. The
densities of MRCs (A) and their exposed apical surfaces (B) were separately
quantified, and the ratio of active MRCs over total MRCs was calculated as
described in the Materials and methods. Scale bars, 50 µm.