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Fig. 7. (A) Evoked excitatory junction potentials (EJPs) recorded from larval nerve–muscle preparations of WT, null mutant CspU1oc, and cDna1–, Ssp–, Clp–, L{Delta}8–, C{Delta}27 transformed animals in CspU1oc null mutant background. Data were pooled for at least two independent transgene insertions except for L{Delta}81 and L{Delta}82, which differed significantly (see Results). The two independent C{Delta}27 transformants display normal transgene expression, indicating that this construct does not fully rescue the larval temperature-sensitive phenotype, whereas adults of these lines show no obvious defects. (A) Sample traces at permissive (18°C) and non-permissive (32°C) temperatures. (B) Evoked EJP amplitudes (mean ± S.E.M.) of (N) preparations.





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