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Fig. 1. Photomicrographs showing whole-mount preparations of eel dorsal aorta (A,C) and rat aorta (B,D) following processing for NADPH diaphorase histochemistry (A,B) and endothelial nitric oxide synthase (eNOS) immunohistochemistry (C,D). In the rat aorta, punctate eNOS-positive staining (arrowheads) occurred around the nuclei (arrows) of the endothelial cells, which was demonstrable with both techniques (B,D). In contrast, no eNOS-positive staining was observed around the nuclei of the endothelial cells in the eel dorsal aorta (A,C). Scale bars, 10 µm.





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