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Fig. 1. Representative data plots of temperature (grey, upper lines) and
CO2 production (black, lower lines) from Pringleophaga
marioni caterpillars exposed to freezing treatments at (A)
–5.8°C (in which all caterpillars survive), (B) –6°C
(which results in caterpillars being left moribund and then dead) and (C)
–18°C (after which no caterpillars show muscle tone or response to
stimuli). Breakpoint (b) and temperature of crystallisation
(Tc) are marked. Small cycles in the CO2 trace
shown in B are a result of minor temperature fluctuations at the IRGA.
Whenever the range of these fluctuations exceeded the mean, the data were
discarded. The break in the middle graph represents a change in data files (we
were restricted by the memory that the acquisition software was able to allot
to data gathering), and the slight difference in trace afterwards (equivalent
to a maximum of 2.1 µW) is a result of re-baselining mid-data-acquisition.
The masses of the caterpillars used in the presented data were 0.2342 g (A),
0.1977 g (B) and 0.3826 g (C), accounting for the variation in the scale of
the right-hand y-axes.
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