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Fig. 2. (A) Ni concentrations in various tissues of juvenile rainbow trout following 42 days of exposure to either control (open bars), 384 µg Ni l–1 (hatched bars) or 2034 µg Ni l–1 (filled bars). Values are means ±1 S.E.M. (N=6–10). Asterisks indicate significant difference (P<0.05) from control mean by a one-way ANOVA with a two-sided Dunnett's post-hoc multiple comparison test. (B) Relative distribution of accumulated Ni among eight tissues in a hypothetical 1-kg rainbow trout. Accumulated Ni in each tissue (µg Ni kg–1 tissue; see A) was multiplied by that tissue's relative proportion of total body mass to normalize individual tissue Ni burdens to a hypothetical 1-kg fish. Therefore, normalized accumulation is expressed as µg Ni kg fish–1 for each tissue. (C) Percentage of accumulated tissue Ni burdens that can be explained purely by Ni within the blood plasma perfusing each tissue (see Discussion for details).





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