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Fig. 4. In situ hybridisation localises sbAQP expression in the kidney,
gills and gastrointestinal tract. sbAQP was highly expressed in some of the
kidney tubules (A) and (C). In the gill, expression was detected along the
length of the lamellae with the most intense signal present in the apical
cellular region of lamellae (D,F) where the chloride cells (arrows) were
localised (F). An intense expression was found in the hind-gut in cells
localised in the lamina propria (G) and in cells (arrows) at the
interface between the two muscle layers of the gut (I). No sbAQP expression
was observed in the liver (J) or in tubules (arrowheads) in control sections
of the kidney (B), gills (E) or gut (H). Scale bars, 1 µm. Asterisks
indicate haem deposits in the kidney.