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Fig. 2. (A) Osmotic water permeability (Pf), (B) apparent
glycerol permeability (
) and
(C) urea uptake of Xenopus oocytes expressing sbAQP. Oocytes were
injected with 50 nl of water containing 10 ng cRNA of sbAQP or 50 nl of
distilled water only (control oocytes) 48 h prior to the experiments.
Pf and
were calculated from the time course of osmotic swelling of the oocyte in a
hyposmotic- or glycerol-containing medium and urea transport activity was
measured by [14C]urea uptake. Values are means ±
S.E.M. of 10 oocytes from different batches.
ME, mercaptoethanol.
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