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Fig. 8. Clustering of FLRFamide-immunopositive terminals in the anterior cardiac plexus (ACP) often gives rise to a bark-like appearance of this structure. (A) A brightest pixel projection of 32 optical sections taken at 2.0 µm intervals through a portion of the anterior cardiac nerve (acn) containing the ACP. (B–D) Single optical sections from the projection shown in A. These images were selected as representative of the labeling seen near the top (B; section 6 of 32), center (C; section 18 of 32) and bottom (D; section 29 of 32) of the acn. Note that the FLRFamide immunolabeled terminals that comprise the ACP are concentrated in the peripheral portion of the acn, with essentially no terminals present in the core of the nerve. Scale bar, 100 µm.





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