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Fig. 7. FLRFamide labeling in the anterior cardiac plexus consists of peripherally
located varicosities derived from large diameter fibers. (A) Confocal
micrograph showing the distal termination of the left ACP. Here, several
immunolabeled fibers can be seen to project fine processes toward the
periphery of the anterior cardiac nerve. These fine neurites arborize,
producing numerous clusters of varicosities which are located within or just
below the acn sheath. This image is a brightest pixel projection of
26 optical sections taken at 2.0 µm intervals. (B) A higher magnification
view of a portion of the acn boxed in A. Note that many of the
varicosities are connected together by fine neurites, giving rise to a `beads
on a string'-like conformation. This image is a brightest pixel projection of
24 optical sections taken at 1.0 µm intervals. (C) A further magnified view
of one cluster of FLRFamide containing varicosities boxed in B. Note the
grape-like cluster of terminal varicosities. This image is a brightest pixel
projection of 44 optical sections taken at 0.5 µm intervals. Scale bars,
100 µm (A,B); 25 µm (C).
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