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Fig. 11. Synapsin-like labeling is restricted to FLRFamide-immunopositive terminals
in the anterior cardiac plexus (ACP). Five axons are present in the portion of
the anterior cardiac nerve containing the ACP. Four of the five axons are
FLRFamide immunopositive and contribute innervation to the ACP. To assess
whether the remaining axon contributes to the innervation of the ACPs,
double-immunolabels pairing FLRFamide and synapsin antibodies were conducted.
In all preparations, the synapsin label was found localized in
FLRFamide-immunopositive terminals. Most FLRFamide labeled terminals exhibited
some degree of synapsin staining. In no preparation were any terminals found
that contained only synapsin immunoreactivity. Interestingly, within a given
terminal, the FLRFamide and synapsin labels are often non-uniformly
segregated. (A1–3) and (B1–3) show examples of this localization
from two different preparations. (A1–3) Brightest pixel projections of
19 optical sections taken at 1.0 µm intervals. (A1) FLRFamide
immunoreactivity pseudocolored red. (A2) Synapsin immunoreactivity
pseudocolored green. The optical sections used to produce A1 and A2 were
simultaneously collected from the same focal planes. (A3) A merged image of A1
and A2. Profiles exhibiting only FLRFamide or synapsin immunolabeling appear
red or green, respectively. Structures showing coincidence of FLRFamide and
synapsin labels appear yellow (or shades thereof). (B1–3) Brightest
pixel projection of 12 optical sections taken at 1.0 µm intervals.
Organization and pseudocoloring of B1–3 is identical to that of
A1–3. Scale bar, 50 µm.
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