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Fig. 10. Lucifer Yellow-CH dye (LY) backfill of an anterior cardiac nerve (acn) produces dye-filling in axons that project posteriorly from the superior oesophageal nerves (sons) and anteriorly from the stomatogastric ganglion (STG). (A1–3) LY backfilling of single acns produces dye-filling in two large diameter axons in each of the paired sons. These axons are FLRFamide immunopositive. Several smaller diameter FLRFamide-immunopositive axons are also present in each son. (B1–3) LY backfilling of single acns also produces dye-filling in a single large diameter axon that projects via the stomatogastric nerve (stn) from the STG. This axon is not FLRFamide immunopositive. As is seen in the son, several small FLRFamide-immunopositive axons are present in the stn. (A1–3) Brightest pixel projections of 19 optical sections taken at 1.0 µm intervals through the son. (A1) LY dye-filled axons pseudocolored green. (A2) FLRFamide immunoreactivity pseudocolored red. The optical sections used to produce A1 and A2 were simultaneously collected from the same focal planes. (A3) A merged image of A1 and A2. Profiles exhibiting only LY dye-filling or FLRFamide immunolabeling, appear green or red, respectively. Structures showing colocalization of LY dye and FLRFamide label appear yellow (or shades thereof). (B1–3) Brightest pixel projection of 22 optical sections taken at 1.0 µm intervals through the stn. Organization and pseudocoloring of B1–3 is identical to that of A1–3. A and B are from the same preparation. Scale bar, 50 µm

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