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Fig. 7. Normal expression pattern of Broad (BR) proteins in the prothoracic gland
(PG) cells. Third instar larvae or white puparia were dissected at designated
times (hours after egg laying). Each ring gland was double stained with
antibodies against BR core region and one of the BR isoforms as described in
Materials and methods. Note that the anti-BR-Z2 antibody also nonspecifically
stained the nucleoli.