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Fig. 4. Critical weights for pupariation for w1118; hs-Z3;
hs-Z3 larvae. Larvae were raised on standard diet, then given (or not
given) a heat shock at 5 h after ecdysis into the second instar. Individuals
of various ages (16–35 h after the heat shock) were weighed, then were
either starved or fed 20-hydroxyecdysone (20E) diet. Pupariation was scored
for each group of treatment. The percentage of pupariation was calculated from
groups of larvae whose weight was the designated point ± 0.025 mg. The
number of animals used for measuring the critical weights for normal L3
pupariation (triangles), L2 prepupae (squares) and competence to form L2
prepupae (circles) was 80, 117 and 99, respectively.
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