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Fig. 3. Overexpression of BR-Z3 during the critical feeding period for the
second-to-third instar molt causes a prolonged second instar and the formation
of L2 prepupae. (A) Second instar larvae were allowed to feed on standard diet
for a designated period and were then transferred to glucose diet (protein
starvation). Ecdysis to the third instar was scored. Each point (circle)
represents a mean of four repetitions (N=16, 8, 8, 8) ±
standard deviation (S.D.). Note the
ET50 for the critical feeding period is
6.5 h. (B)
BR-Z3 was overexpressed by a heat shock at a designated time in the second
instar, and the animals were maintained on the standard diet. The L2 prepupae
were scored. Each point (diamond) represents a mean of four repetitions
(N=25, 10, 10, 10) ± S.D. Note that the
ET50 of the induction of L2 prepupae is
5.8 h after
ecdysis into the second instar.