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Fig. 2. nNOS mRNA expression in the brain of embryonic zebrafish at different
developmental stages. (A-C) Whole mounts; (D–J) cryosections;
(H–J) comparison with AT immunoreactive neurons in corresponding regions
at a representative stage (24 h.p.f., cryosections). (A,B) 19 h.p.f.; nNOS
mRNA expression in the forebrain, in two bilateral cell populations (arrows)
as part of vrc (sagittal view in A and frontal view in B). (C) 23 h.p.f.; the
bilateral vrc cell clusters (arrow and arrowhead in whole-mount preparation)
are seen in a semi-sagittal view. (D) 24–25 h.p.f.; one nNOS
mRNA-expressing vrc cell (blue) is shown adjacent to the initial pigmentation
of the retinal epithelium (brown; RPE, arrowheads) from a frontal view. (E,F)
30 h.p.f.; nNOS mRNA-expressing cell bodies in vcc (E; sagittal view) and nNOS
mRNA-expressing cell populations in drc (F; sagittal view). (G) 34 h.p.f.;
nNOS mRNA-expressing cells in hc (sagittal view). (H–J) AT
immunoreactivity at 24 h.p.f. in brain regions corresponding to sites of the
nNOS mRNA expression in vrc and vcc (H, sagittal view; I, frontal view), and
in hc and medulla (J; sagittal view). Scale bars: in A, 100 µm (A,B); 30
µm (C,F); in D, 20 µm (D,E,G); in H, 50 µm (H–J). V,
ventricle.
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