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Fig. 3. Differential interference contrast (DIC) and fluorescence images of
dispersed gill cells from freshwater rainbow trout (A–C) and
seawater-adapted (30
) trout (D–F). Cells were obtained from the
mitochondria-rich (MR) 1.05–1.09 g ml–1 Percoll layer
and stained with Mitotracker Green-FM (100 nmol l–1, 20 min;
B,E) or labelled with PNA Alexa fluor 594 (40 µg ml–1;
C,F) for identification of MR and PNA+ cell types, respectively.
All pictures in A–C and D–F are of the same field of view. Scale
bar, 10 µm.
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