spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Elution profile of heparin–Sepharose affinity chromatography column illustrating the elution of protein (absorbance at 280 nm;solid symbols) and the concentration of NaCl in the eluate (open symbols). Typical results from a single experiment. Horizontal bars indicate those fractions with detectable lipase activity against TAG substrates. Activity eluting early in the column is HSL, which is not retarded by the heparin affinity column. Activity eluting later in the chromatogram at high salt concentration is LPL, which is retarded by the heparin affinity column.





Right arrow Return to article