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Fig. 1. Schematic diagram of the experimental protocol used to confirm that the
in situ trout hearts were experiencing severe hypoxia. One group of
in situ hearts was perfused with severely hypoxic saline
(N=8), while a second group of hearts was perfused with severely
hypoxic saline containing 1.5 mmol l–1 of sodium cyanide
(NaCN) (N=7). Time intervals are marked in min above the protocol.
The solid line represents the end-diastolic pressure developed by the
ventricle. POUT was set to a physiologically realistic
level of 50 cmH2O. The steps identify the maximum cardiac output
tests (
MAX), where
PIN was raised sequentially from 3 cmH2O to 4
cmH2O, and finally to 4.5 cmH2O. During all periods of
oxygenated cardiac perfusion, was
maintained at a physiologically resting level of 16–17 ml
min–1 kg–1, by adjusting
PIN as needed. The shaded rectangle represents the period
of severe hypoxia (PO =5–10 mmHg). During hypoxia,
PIN was not adjusted and
was allowed to fall.
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