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Fig. 6. (A) Steady-state activation and inactivation of putative K+
currents of 4–6 d.p.f. zebrafish inner muscle. Steady-state activation
(filled circles; N=7) was determined by applying a series of 5 ms
activating pulses from a holding potential of –100 mV to a range of
potentials from –45 to +65 mV at 5 mV intervals (inset, right). These
pulses were followed immediately by a step to –130 mV to enable the
measurement of tail currents. The amplitudes of the tails were normalised to a
maximum value of 1 and plotted against the amplitude of the activating pulse.
Steady state inactivation (open circles; N=6) was determined as in
Fig. 3C (inset, left). (B)
These outwardly directed currents are blocked by 10 µmol
l–1 4-aminopyridine (4AP) in a use-dependent manner. Stepwise
250 ms depolarizations from a holding potential of 100 mV to a potential of 0
mV were applied as the larva was perfused in saline containing 10 µmol
l–1 4AP. Numbers indicate the sequence of depolarizing
pulses.
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