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Fig. 5. Isolation of K+ currents from inner and outer muscle of
4–6 d.p.f. zebrafish larvae was performed by applying depolarizing
pulses to voltage-clamped fibres in saline in which all sodium ions were
replaced with equimolar choline ions and all calcium ions were replaced with
equimolar cadmium ions and supplemented with BAPTA (10 mmol
l–1). (A) K+ currents of outer muscle. A series of
250 ms depolarizations from a holding potential of –100 mV to a range of
potentials from –95 to 25 mV evoked outwardly directed currents with
little or no evidence of inactivation. (B) A current–voltage plot of
such currents shows that they appear at membrane potentials more positive to
around –40 mV and increase with more positive potentials. (C)
K+ currents of inner muscle. (D) The current–voltage plot of
these currents reveals that they are activated at potentials more positive
than around –20 mV. There is still some residual inward current. Values
are means ± S.E.M. of at least eight separate
experiments
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