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Fig. 3. Diuretic hormone 44 (DH) activation of CG8422-expressing HEK-293 cells produces a dose-dependent increase in CRE-luciferase activity. Dose-response curve for DH activation of CRE-luc gene expression in cells transiently co-expressing CG8422. The calculated EC50 is 1.47 nmol l-1. Values are means ± S.E.M. and represent the results pooled from three experiments that were performed in triplicate. Values observed with exposures to DH above 5x10-10 mol l-1 were statistically different from values observed with exposure to vehicle alone.





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