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Fig. 1. HEK-293 cells expressing CG8422 display ßarr2-GFP translocation in response to diuretic hormone 44 (DH) neuropeptide. A 10-min time series imaging a single CG8422-expressing cell before and following exposure to 10-6 mol l-1 DH (final concentration). Numbers at the bottom right of each panel refer to minutes after DH exposure. Prior to peptide application, most of the fluorescence is uniformly distributed in the cytoplasm. Within 1 min, and at times thereafter, the fluorescence translocates to the cell membrane. Similar results were obtained from three independent transfections: the majority of GFP-positive cells displayed clear ß-arrestin translocation within minutes of exposure to DH.





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