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Fig. 5. Optical responses in the antennal lobe (AL) elicited by electrical
stimulation of the medial nerve (MN) and the lateral nerve (LN). (A) Real
image of the moth brain (boxed area of inset). The boxed area in the schematic
drawing corresponds to the MOS-type image sensor used by optical recording.
Scale bar, 500 µm. OL, optic lobe; PC, protocerebrum. (B) Optical responses
in the AL. All optical images were superimposed on the bright real image of
the AL. Consistent response patterns in the macroglomerular complex (MGC) were
evoked by the electrical stimulation of the medial nerve (MN) or the lateral
nerve (LN). The pattern was initially a depolarization of the antennal nerve
(AN; 3.6–7.2 ms after the onset of the stimulation) and, subsequently, a
depolarization of the MGC (4.8–9.6 ms). At 7.2 ms from the stimulus of
the MN, the area strongly (>0.4% of the background fluorescence) responding
to stimulation of the MN was restricted to the medial half of the MGC. By
contrast, the area strongly (>0.4% of the background fluorescence)
responding to stimulation of the LN was restricted to the lateral half of the
MGC. (C) Time course of the optical signals in the AL evoked by stimulation of
the MN (upper panel) and the LN (lower panel). The optical signal was
calculated by averaging signals recorded in areas that had a response of
>0.3% (–
F/F) at 7.2 ms from the stimulus
onset and filtered at 246 Hz (Fc). The response, evoked in
the MGC, had a peak at 7.2 ms after the stimulus onset. The response had
another slow component (arrowheads) after the first peak of the
depolarization. The annodal break was visible just after the stimuli (S).
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