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Fig. 3. Nucleotide sequence and deduced amino acid sequence of S.
haematocheir OHSS. Nucleotides are numbered to the left from the first
base at the 5' end, and amino acids are numbered to the right from the
initiating methionine. The putative signal peptide sequence is shown in bold.
The boxed amino acids indicate determined sequences. The putative cleavage
site accompanying activation is shown by a solid triangle. The catalytic triad
of the serine protease domain is enclosed by a circle. A putative
polyadenylation signal is doubly underlined. Putative N-glycosylation sites
are indicated by asterisks. Locations of three introns (Int-1, 240 bp; Int-2,
316 bp; and Int-3, 842 bp) are marked by upward-pointing arrows. Positions of
nucleotide primers used for cloning and expression analysis of OHSS gene are
indicated by red arrows (see text for details).