spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Final step of purification of S. haematocheir OHSS. (A) RP-HPLC analysis of the proteins. The active fractions eluted by molecular-sieve chromatography (gel filtration) were concentrated, and the proteins were eluted with a linear gradient of 8%–52% acetonitrile containing 0.1% HCl over 80 min (flow rate at 0.6 ml min–1). Numbers (1–6) indicate peaks of protein. (B) OHSS biological assay of fractions eluted by FPLC (gel filtration) and RP-HPLC. Bioassay carried out for 1.5 h with two ovigerous setal segments per fraction (see Saigusa and Iwasaki, 1999). C, control assay in distilled water. Values are means ± S.D. (C) Protein analysis by SDS-PAGE. The polyacrylamide gel was stained with Coomassie Brilliant Blue. The marker proteins were ovalbumin (45 kDa), carbonic anhydrase (30 kDa), trypsin inhibitor (20.1 kDa), and lysozyme (14.3 kDa; Amersham). Two bands (25 and 22 kDa) appeared in fractions 3–5, while a single band (25 kDa) appeared in fraction 6.





Right arrow Return to article