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Fig. 9. Effect of inside-negative vesicular interior on (A)
[14C]oxalate/Cl exchange and (B)
36Cl/Ox2 exchange in
hepatopancreatic BBMV. (A) Vesicles were preloaded with 100 mmol
l1 KCl and 100 mmol l1 TMA-gluconate and
incubated in media containing 0.1 mmol l1
[14C]oxalate and either 100 mmol l1 K-gluconate
and 100 mmol l1 TMA-gluconate (open bar) or 200 mmol
l1 TMA-gluconate (hatched bar). (B) Vesicles were preloaded
with 50 mmol l1 K2SO4 and 75 mmol
l1 TMA-gluconate and incubated in media containing 10 mmol
l1 36Cl and either 100 mmol
l1 K-gluconate and 100 mmol l1
TMA-gluconate (open bar) or 200 mmol l1 TMA-gluconate
(hatched bar). All media contained 40 mmol l1 Hepes-Tris at
pH 7.0.