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Fig. 9. Effect of inside-negative vesicular interior on (A) [14C]oxalate/Cl exchange and (B) 36Cl/Ox2– exchange in hepatopancreatic BBMV. (A) Vesicles were preloaded with 100 mmol l–1 KCl and 100 mmol l–1 TMA-gluconate and incubated in media containing 0.1 mmol l–1 [14C]oxalate and either 100 mmol l–1 K-gluconate and 100 mmol l–1 TMA-gluconate (open bar) or 200 mmol l–1 TMA-gluconate (hatched bar). (B) Vesicles were preloaded with 50 mmol l–1 K2SO4 and 75 mmol l–1 TMA-gluconate and incubated in media containing 10 mmol l–1 36Cl and either 100 mmol l–1 K-gluconate and 100 mmol l–1 TMA-gluconate (open bar) or 200 mmol l–1 TMA-gluconate (hatched bar). All media contained 40 mmol l–1 Hepes-Tris at pH 7.0.





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