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Fig. 1. In vitro microperfusion of Malpighian tubules of the yellow fever mosquito, Aedes aegypti. (A) Perfusion of a tubule segment containing a stellate cell. (B) Perfusion of a tubule segment without stellate cells. The lumen of Malpighian tubules was cannulated with a double-barreled glass pipette for perfusion of the tubule lumen and for measurements of the transepithelial voltage (Vt) and resistance (Rt). Vl is the voltage measured at the distal end of the perfused segment. A current (I=50 nA) was injected into the tubule lumen when measurements of Rt were desired. BB, brush border; PC, principal cell; SC, stellate cell; SJ, septate junction. (C) Simple electrical equivalent circuit of transepithelial electrolyte transport across Malpighian tubules. Ec, electromotive force of the active transport pathway; I, intraepithelial current; Rc, transcellular resistance; Rsh, shunt resistance. Rt is the total resistance of Rc and Rsh in parallel.





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