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Fig. 1. Determination of critical oxygen partial pressure (Pc). This is the raw data file from an individual grasshopper, showing CO2 emission (solid line) and atmospheric O2 (broken line). As oxygen partial pressure (PO2) drops incrementally, CO2 transiently increases. Briefly, we compared 95% confidence intervals constructed around the mean CO2 p.p.m. in each box. Box A is the PO2 where CO2 was significantly less than at the next higher PO2 (box B) and less than the average CO2 at all higher PO2s (box C).





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