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Fig. 3. In vivo effect of estradiol (E2) and E2 administered in combination with melatonin (mel) on mast cell number (MCN) in the frog Rana esculenta. All the animals were injected with E2 24 h before being sacrificed and were killed at 48 h, 72 h and on day 8 after the injection of melatonin or Krebs Ringer bicarbonate (KRB) buffer (control). Values are expressed as means ± S.D. Different letters indicate significance (a versus b, c, d, P<0.01; b versus d, e, P<0.01; b versus c, P<0.05; c versus d, e, P<0.01).





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