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Fig. 6. Work loops measured under conditions that maximized power output [blue, phase of activation: 0.34; mean length: 9.81 mm (1.05Lop); peak-to-peak strain amplitude: 0.95Lop; power output: 91.0 W kg–1] and under conditions that replicated conditions measured in vivo during tethered flight [red, phase of activation; 0.52, mean length: 9.81 mm (1.05Lop); peak-to-peak strain amplitude: 0.77Lop, power output: 53.9 W kg–1]. Time progresses in each loop in a counterclockwise direction, as indicated by the arrowhead on each loop. The filled circle on each loop indicates the time of muscle activation. Both work loops are from Moth 1 in Tables 1 and 2. Activation of the muscle prior to the onset of shortening maximized power output. Under in vivo conditions in which muscle activated occurred near the onset of shortening, the muscle generated relatively low force during shortening and did not relax completely prior to lengthening.





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