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Fig. 5. Power output of the dl1 muscles is plotted over a range of activation phases, experimental lengths and strain amplitudes that encompass both peak power and in vivo operating conditions. (A–C) Variation in mechanical power output as a function of experimental length. Experimental length is normalized to operating length. Each plot shows measurements taken at one peak-to-peak strain amplitude normalized to Lop: (A) 0.048, (B) 0.083, (C) 0.115. Within each plot, each curve shows data from measurements at one of five values of the phase of activation: {blacktriangledown} 0.31, {diamondsuit} 0.37, {blacksquare} 0.42, {blacktriangleup} 0.47, • 0.52. At each amplitude power output was consistently lowest at the phase values (0.47 and 0.52) that were closest to the phase of activation measured in vivo (0.49±0.04; Tu and Daniel 2004). (D–F) Contour plots showing power output, coded by color, as a function of the phase of activation and experimental length. Each plot is based on 90 measurements of power taken at five values of activation phase and 18 values of experimental length. The contour lines were fitted to the data by interpolation using an inverse distance method (griddata.m, Matlab v.4, The Math Works Inc., Natick MA USA). Each plot shows measurements taken at one peak-to-peak strain amplitude, normalized to Lop: (D) 0.048, (E) 0.083, (F) 0.115. At all three amplitudes, power output near the values of operating length and phase of activation measured in vivo (open circle) was substantially lower than the peak power output. The mean strain amplitude measured in vivo (0.090±0.031Lop) lies between the amplitudes tested in B,C, and E,F. Data in A–F are from one muscle preparation (Moth 6 in Tables 1, 2).





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