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Fig. 7. Functional assessment of B. taurus {alpha} crystallin after 1 h incubation at 60°C in the presence of D. mawsoni {gamma} crystallin. (A) Experimental {Delta} mg B. taurus {alpha} crystallin + 1 mg D. mawsoni {gamma} crystallin (x) and control reaction of {Delta} mg B. taurus {alpha} crystallin + 1 mg B. taurus {gamma} crystallin (x). After 1 hsamples were removed from the cuvette and any precipitate was removed by centrifugation at 21 000 g at 4°C in a benchtop microcentrifuge. (B) Supernatants (~750 µl) were then transferred to a new cuvette and a further 1 mg of B. taurus {gamma} crystallin was added. B. taurus {gamma} crystallin was concentrated such that the total volume in the second assay (after addition of the B. taurus {gamma} crystallin) was not greater than 1 ml. Final composition of assay was (B. taurus {alpha} + D. mawsoni {gamma}) supernatant + B. taurus {gamma} crystallin (x) for the experimental and (B. taurus {alpha} + B. taurus {gamma}) supernatant + B. taurus {gamma} crystallin (x). Second assay incubation was for 1 h at 60°C.





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