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Fig. 5. Chaperone protection by 
crystallin of heat-aggregated 
crystallin (A–C) along with protection of DTT-denatured lysozyme
(D–F). (A,D) D. mawsoni; (B,E) T. obesus; (C,F) B.
taurus. Temperatures for the assay are indicated in each panel. Values
are means ± S.E.M. (N=3). In A–C the
crystallin and crystallin are both from the same species, with
fractional amounts of crystallin added based on a 1:1 mass ratio of
to crystallin at a final assay concentration of 1 mg
ml–1 for both crystallins (i.e. x in A–C). In
D–F the amount of crystallin added was based on a 1:1 mass ratio
of crystallin to lysozyme, where in all assays (D–F) there was
0.2 mg ml–1 of lysozyme containing 20 mmol
l–1 DTT. For A–C: x ; x 1:16
:; x 1:8 :; x 1:4 :;
x 1:2 :; x :. For D–F: x
0:1 :lyso; x 1:1 :lyso; x 2:1 :lyso; x
3:1 :lyso; x 2.5:1 :lyso; x 5:1 :lyso;
x 7.5:1 :lyso; x 10:1 :lyso; x 11:1
:lyso.
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