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Fig. 4. Determination of the upper limit of thermal stability TS for the (A) {alpha} and (B) {gamma} crystallins from D. mawsoni, measured as the change in turbidity (A360) over time at various incubation temperatures. Concentration of crystallins was 1 mg ml–1 in all assays. (A) Upper limit of TS for {alpha} crystallin. At temperatures above 47°C, turbidity increases with time. {alpha} at 47°C (x); 55°C (x); 60°C (x). (B) Upper limit of TS for {gamma} crystallin. {gamma} at 30°C (x); 33°C (x); 35°C(x); 42.5°C (x); 44°C (x); 45°C (x); 47°C (x); 57.5°C (x). The TS was determined as 47°C for the {alpha} and 33°C for the {gamma} crystallin.





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