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Fig. 4. Determination of the upper limit of thermal stability
TS for the (A)
and (B)
crystallins from
D. mawsoni, measured as the change in turbidity (A360)
over time at various incubation temperatures. Concentration of crystallins was
1 mg ml1 in all assays. (A) Upper limit of
TS for
crystallin. At temperatures above 47°C,
turbidity increases with time.
at 47°C (x); 55°C
(x); 60°C (x). (B) Upper limit of TS for
crystallin.
at 30°C (x); 33°C (x);
35°C(x); 42.5°C (x); 44°C (x); 45°C
(x); 47°C (x); 57.5°C (x). The
TS was determined as 47°C for the
and 33°C
for the
crystallin.