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Fig. 6. Real-time measurement of the [Ca2+]i response by A. stephensi leucokinin receptor. S2 cells were co-transfected with the A. stephensi leucokinin receptor ORF and apoaequorin ORF constructs, and expression induced. Peptide was injected at 15 s. Samples were stimulated with either Anopheles leucokinin I (A), II (B) or III (C) peptide at a concentration of 10–7 mol l–1. (D) Comparison of the responses to the three Anopheles leucokinin peptides applied at a concentration of 10–7 mol l–1. Data are expressed as [Ca2+]i (nmol l–1) against time (s); measurements were taken at 0.1 s intervals. The traces shown are average responses ± S.E.M. (N=8). Error bars are negligible for all panels.





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