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Fig. 11. Anopheles leucokinin receptor is expressed in stellate cells of
the Malpighian tubule. Tubules were stained with anti-Anopheles
leucokinin peptide purified IgG, raised as described in the text. Texas Red
secondary antibodies were used to visualize the primary antiserum and DAPI was
used to stain nuclei (blue). (A,B), Fluorescence microscopy of immunostained
whole-mount tubules revealed staining in the secondary stellate cell type
(arrows), concentrated in the basolateral membrane. (C) Tubules were processed
as for A and B, but with pre-immune serum, confirming the specificity of the
antibody. Only low-level non-specific staining of apical microvilli was
observed. All images were captured on a Zeiss 510 Meta confocal microscope
using a 63 x objective and the approximate scale can be determined from
the tubule diameter, which can be taken to be 35 µm.
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