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Fig. 6. Immunoblot analysis of bacterial expressed Aedes CA. (A) Protein
staining (Fast Green) of an electroblot from an SDS-PAGE analysis of extracts
from cultures of bacteria that had been transformed with the CA expression
vector. Labels at the bottom of the panel show the molecular mass marker lane
(M) and extracts from 3 or 24 h of culture time. Labels on the left indicate
molecular masses of the markers. A protein of approximately 35 kDa displays
the highest level of expression. In (B) the same blot was subsequently
immunostained with the XPress epitope antibody (Invitrogen, lane XP) preimmune
serum from rabbit 33 (left lane labeled PI) or rabbit 34 (right lane labeled
PI) or the antisera from the rabbits (lanes 33 and 34). Note intense labeling
of the same band identified by the XPress antibody as detected by the two
rabbit antisera to the CA peptide at approximately 35 kDa, the expected mass
of the expressed recombinant CA protein (arrow).
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