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Fig. 1. Simplified diagram of the respirometry system. Not to scale. Data acquisition system not shown. Oxygen and nitrogen tanks are at the bottom; each opens into a manifold through which oxygen or nitrogen flow can be controlled with a needle valve (NV). MFC, mass flow control valve (associated controller not shown). Dashed rectangle is temperature-controlled cabinet (Peltier effect; PE-temp). S, scrubber (H2O and CO2); EQ, equilibration coil; RC, respirometry chamber; T, thermocouple; H2O, water vapor analyzer; CO2, carbon dioxide analyzer. See text for details.





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