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Fig. 2. ENpa and GSPRFFamide depress the contraction of the vasoconstrictor muscle
evoked by the stimulation of the pericardial nerve. (A) Effects of
10–7 mol l–1 ENpa (Ai) and
10–7 mol l–1 GSPRFFamide (Aii) on the
contraction evoked by a train of the electrical pulse (1 ms, 1.6 V, 10 Hz) for
1 s. The stimulus train was applied every 20 s. Peptides were applied for 2
min as indicated by bars. Ai and Aii are from the same preparation. (B)
Concentration–response relationships of the effects of ENpa and
GSPRFFamide. Amplitude of the most depressed contraction after the peptide
application is normalized to that of the control contraction and the mean
± S.E. was plotted against the concentration of the peptides
(N=3
6). (C) Time-dependent effects of the peptide actions.
Amplitude of the muscle contraction was normalized to the control contraction
just before the peptide application and plotted against the time
(N=3). 10–5 mol l–1 of ENpa or
GSPRFFamide was applied for 2 min as indicated.
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