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Fig. 3. (A) Sets of phosphorescence intensity images of a 1.4 mm Hb-poor
Daphnia magna taken at seven different delay times
(td) under 0.5% (top) and 19.6% air saturation (bottom),
respectively. From all phosphorescence images, a background image acquired at
td=3000 µs has been subtracted. For orientation, an
image of the animal taken under transmission illumination is shown at the
left. In the top sequence, the limbs appear in sharp contours because the
animal had stopped its limb beating activity owing to the low oxygen
conditions. Note the strong phosphorescence signal in the region of the shell
gland (arrow). (B) For the heart region, i.e. the image areas marked by white
ellipses in A, the integrated phosphorescence intensity (Z) was
plotted against td. Exponential decay curves (solid lines)
were fitted according to Equation
3 to the data that were measured at 0.5% (filled circles) and
19.6% air saturation (open circles). a.u., arbitrary units.
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