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Fig. 11. The myonuclei content of isolated single fibres from (A) a slow muscle fibre 27 µm in diameter and (B) a fast muscle fibre 157 µm in diameter. The images represent a computed reconstruction of a z-series of 1 µm confocal sections through the fibre. The nuclei (green) were visualised by staining with SYTOX green®. (C) The relationship between muscle fibre diameter (µm) and the number of nuclei per cm in single fibre segments isolated from the slow (red symbols) and fast (black symbols) myotomal muscle from the DB morph (open circles), the LB-morph (filled circles), the PL morph (open triangles) and the PI morph (filled triangles). Fibres were isolated from 4–6 individuals per morph in the size range 20–27 cm FL. The fitted lines represent second order polynomials with the following equations. Slow muscle: nuclei cm–1=23.38+34.76 (fibre diameter)+0.043(fibre diameter)2 (Adj. r2=0.65; residual d.f.=247; P<0.0001). Fast muscle: nuclei cm–1=231.80+9.00 (fibre diameter)+0.098 (fibre diameter)2 (Adj. r2=0.92; residual d.f.=620; P<0.0001).





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