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Fig. 4. The effects of acetylcholine and pituitary adenylate cyclase-activating polypeptide (PACAP) on smooth muscle preparations from adult zebrafish intestine. (A) Acetylcholine increased mean force and amplitude of contractions in both longitudinal (filled squares) and circular (open squares) preparations of the middle intestine. (B) PACAP-27 decreased the mean force and amplitude in circular strip preparations of middle intestine, after blocking nitric oxide synthase formation by L-NAME (which increased the mean force and amplitude). (C) PACAP-27 increased mean force in longitudinal preparations of the middle intestine. cm, circular muscle layer; lm, longitudinal muscle layer.





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