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Fig. 3. The effects of PACAP (pituitary adenylate cyclase-activating polypeptide) on gut motility in zebrafish larvae over successive time periods (in vivo experiments). In the control experiment (A) the injection of saline (NaCl) did not affect the frequency of anterior anterograde waves (cycles min-1). At 5 (C) but not 4 d.p.f. (B) the injection of PACAP (20 nl, 10-6 mol l-1) reduced the frequency of the anterior anterograde waves, indicating the presence of functional PACAP receptors.





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